Journal: Oncoscience

Article Title: MDA-9/Syntenin regulates differentiation and angiogenesis programs in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: (A) Effective knockdown of MDA-9/Syntenin was evident in 3 established HNSCC cell lines. Actin was used as a loading control. Marked downregulation of cell cycle regulatory molecules CCND1 and CDK4 (B), activated STAT3 (Tyr705, C) was evident by Western-blot analysis in the MDA-9/Syntenin depleted HNSCC cells as indicated. Decreased expression of PI3K (D) and CTNNB1 (E) was also evident by Western-blot analysis in the MDA-9/Syntenin depleted HNSCC cells.

Article Snippet: The EGFR, CCND1, CDK4/6, CTNNB1, PI3K, STAT3, EF1-alpha and Beta-actin antibodies were procured from Cell Signaling (dilution 1:200).

Techniques: Knockdown, Control, Western Blot, Expressing

Journal: Oncoscience

Article Title: MDA-9/Syntenin regulates differentiation and angiogenesis programs in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: (A) MDA-9/Syntenin overexpression in the OKF6 cells as indicated. (B) Overexpression of MDA-9/Syntenin in OKF6 cells resulted in a significant increase in proliferation (p=0.01), compared to the empty vector treated group. (C-E) Marked upregulation of CCND1 (C), CDK4, PI3K (D), activated STAT3 (Tyr705) and CTNNB1 (E) in the MDA-9/Syntenin-transfected cells compared to the empty vector-treated cells as indicated. EF1-alpha was used as a loading control. (F) Co-immunoprecipitation of MDA-9/Syntenin and CTNNB1 in all but one oral epithelial cell lines. IgG: Control immunoglobulin G, Lys: Whole cell lysate used for IP analysis. IB: Immunoblotting; IP: Immunoprecipitation.

Article Snippet: The EGFR, CCND1, CDK4/6, CTNNB1, PI3K, STAT3, EF1-alpha and Beta-actin antibodies were procured from Cell Signaling (dilution 1:200).

Techniques: Over Expression, Plasmid Preparation, Transfection, Control, Immunoprecipitation, Western Blot